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ลายพิมพ์ดีเอ็นเอของชาสกุล Camellia จากลาดับนิวคลีโอไทด์บางส่วนของยีน Chalcone synthase (DNA Fingerprinting of The Genus Camellia Based on Partial Sequences of Chalcone synthase) |
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| รหัสดีโอไอ | |
| Creator | จิตรอนงค์ คารศ |
| Title | ลายพิมพ์ดีเอ็นเอของชาสกุล Camellia จากลาดับนิวคลีโอไทด์บางส่วนของยีน Chalcone synthase (DNA Fingerprinting of The Genus Camellia Based on Partial Sequences of Chalcone synthase) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 230 |
| Keyword | DNA fingerprint, Camellia, ChS, Chalcone synthase, RFLP |
| ISSN | 8578664 |
| Abstract | DNA fingerprint of the tea plant genus Camellia based on Chalcone synthase (ChS) sequences, was investigated. The partial 768-bp ChS gene sequences that could be achieved by PCR (Polymerase Chain Reaction) were retrieved from GenBank (as of March, 2012). Sixty -four data were then analized by RFLP (Restriction Fragment Length Polymorphism). Eight , 6, 7 and 3 different DNA profiles were detected after digestion of the ChS gene with AciI, BbvI, HpaII andRsaI, respectively. Later, 27 characteristic RFLP patterns were identified for all 64 samples using these composite digestive enzymic profiles. Of these, the restriction endonuclease patterns are able to differentiate between C. sinensis, C. sinensis var. assamica and C. Oliefera; the typical varieties of tea that planted extensively throughout the North of Thailand. |
