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ความหลากหลายทางพันธุกรรมของยางพารา (Heveabra siliensis Muell Arg.) จากลำดับเบสบางส่วนของยีน Cinnamyl Alcohol Dehydrogenase (Genetic Diversity of The Para Rubber Tree (Heveabra siliensis Muell Arg.) Based on Partial Sequences of Cinnamyl Alcohol Dehyd |
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| รหัสดีโอไอ | |
| Creator | อรวรรณ วราพุฒ |
| Title | ความหลากหลายทางพันธุกรรมของยางพารา (Heveabra siliensis Muell Arg.) จากลำดับเบสบางส่วนของยีน Cinnamyl Alcohol Dehydrogenase (Genetic Diversity of The Para Rubber Tree (Heveabra siliensis Muell Arg.) Based on Partial Sequences of Cinnamyl Alcohol Dehydrogenase Gene) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 218 |
| Keyword | rubber tree, cinnamyl alcohol dehydrogenase, Heveabra siliensis |
| ISSN | 8578664 |
| Abstract | Para rubber tree (Heveabra siliensis Muell Arg.) is an important economic crop of Thailand. The bark of rubber tree has been widely used as raw materials by wood-processing industries or used directly in a production of furniture which is currently in high demand. Cultivar selection of the rubber tree, in particular associated with wood characteristic have been reported which is typically based on morphological criteria. Nevertheless, this method requires specialists and is very time-consuming. In such cases genetic sequences, the Cinnamyl alcohol dehydrogenase (CAD) gene involved in lignin biosynthesis in plants, and particular PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) method can provide specificity, accuracy and fast as an alternative means of identification to the cultivar level. In this study, therefore, eight cultivars of rubber tree, RRIM 600, RRIT 251, BPM 24, Chachoengsao 50, PB 260, PB 255, PB 235 and Bangpid were examined. The extracted DNA was PCR-amplified and the obtained CAD gene (1,281bp in length) was then cloned and subsequently sequenced. Two restriction endonucleases, ScrFI and MboII, were then selected to produce 8 different DNA restriction digested patterns that were consistent among individuals within each cultivar of the examined rubber tree. |
