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ลายพิมพ์ดีเอ็นเอของยางพาราจากลาดับนิวคลีโอไทด์บางส่วนของยีนcaffeate O-methyl-transferase (DNA Fingerprint of the Para Rubber Trees (Hevea brasiliensis Muell. Arg) Based on Partial Sequences of the Caffeate O-Methyl-Transferase Gene) |
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| รหัสดีโอไอ | |
| Creator | นิจรดา ย๊ะหมื่น |
| Title | ลายพิมพ์ดีเอ็นเอของยางพาราจากลาดับนิวคลีโอไทด์บางส่วนของยีนcaffeate O-methyl-transferase (DNA Fingerprint of the Para Rubber Trees (Hevea brasiliensis Muell. Arg) Based on Partial Sequences of the Caffeate O-Methyl-Transferase Gene) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 262 |
| Keyword | rubber tree, caffeate O-methyl-transferase, DNA fingerprint, PCR-RFLP |
| ISSN | 8578664 |
| Abstract | The Para rubber tree (Hevea brasiliensis Muell. Arg) is an economically important plant of Thailand. Selective breeding of this species needs to monitor in the plantation crop where time is consuming. Moreover, the morphological characterization to identify the rubber tree variety requires specialists. In this study, the partial sequencing of COMT (Caffeate O-Methyl-Transferase) gene involved in lignin synthesis, an important component of wood, was undertaken. The PCR(Polymerase Chain Reaction) and RFLP (Restriction Fragment Length Polymorphism) techniques were used to generate DNA fingerprinting of 10 cultivars of the rubber tree;1) PB 260, 2) RRIT 408, 3) RRIT 226, 4) PB 235, 5) BPM 24, 6) RRIM 600, 7) CHACHOENGSAO (CCO) 50, 8) BANGPID (BP), 9) PB 255 and 10) SONGKHLA (SKA)36. The result showed that the COMT amplified fragments of each rubber tree sample varied from 1,569 to 1,573 bp in length. The cloned COMTamplicons were sequenced and 4 restriction enzymes (BanI, BbsI, BtgZI and MspI) were subsequently selected. Eight different endonuclease digest patterns (A, B, C, D, E, F, G and H) were able to discriminate among those varieties. However, DNA pattern E was not able to distinguish between the rubber tree cultivars PB 235, PB 255 and PB 260. |
