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การค้นหาโปรตีนที่มีปฏิสัมพันธ์กับโปรตีน 14-3-3 จากกุ้งขาว (Litopenaeus vannamei) (Identification of 14-3-3 interacting proteins from white shrimp (Litopenaeus vannamei)) |
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| รหัสดีโอไอ | |
| Creator | ศศิธร บุญประคอง |
| Title | การค้นหาโปรตีนที่มีปฏิสัมพันธ์กับโปรตีน 14-3-3 จากกุ้งขาว (Litopenaeus vannamei) (Identification of 14-3-3 interacting proteins from white shrimp (Litopenaeus vannamei)) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 334 |
| Keyword | 14-3-3 protein, yeast two hybrid, Litopenaeus vannamei |
| ISSN | 8578664 |
| Abstract | The 14-3-3 proteins are a family of conserved regulatory molecules expressed in all eukaryotic cells. In order to investigate biological function of the 14-3-3 protein in shrimp, we performed yeast two hybrid method to screen proteins from shrimp cDNA library interacting with the 14-3-3 epsilon. 10 positive clones were selected and sequenced. Among 10 positive clones, 5 coding genes with known function were obtained, including exosome complex component, hypothetical protein, late cornified envelope protein 2B-like, ribosomal protein S3 and elongation factor 1 alpha. Whereas remaining genes with unknown function were found. These finding is basic knowledge that has led us to understand the function of 14-3-3 epsilon in shrimp. However the interaction between 14-3-3 epsilon and the above-mentioned interacting proteins need to further studies |
