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โปรตีนกระตุ้นเซลล์จับกินสิ่งแปลกปลอมในปลา (Phagocytosis Activating Protein in Fish) |
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| รหัสดีโอไอ | |
| Creator | ภัทรพร คงมี |
| Title | โปรตีนกระตุ้นเซลล์จับกินสิ่งแปลกปลอมในปลา (Phagocytosis Activating Protein in Fish) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 351 |
| Keyword | phagocytosis activating protein (PAP), immune system, carp fish, DNA vaccine |
| ISSN | 8578664 |
| Abstract | The objective of this research is to study the gene of ribosomal protein L26 (RPL26) or phagocytosis activating protein (PAP) of Penaeus monodon that encodes of PAP to stimulate the immune system in shrimp. The findings indicated that there is a homologous of RPL26 gene in the several species of fishes. This research used one of the most the popular fish: common carp fish (Cyprinus carpio) as the sample to be examined. This study consisted simulation the immune system of fish by injecting the inactivated Aeromonas hydrophila then observed the PAP expression by using Reverse-transcription PCR (RT-PCR) method. The result showed that PAP gene had a higher expression in the infected fish compared with normal fish. Additionally, this research also applied biotechnology methods to produce the recombinant plasmid (DNA vaccine): PAP-phMGFP and injection into carp fish at the 20, 40, 80 ?g / per fish. After 3 and 7 days of injection, the expression of the recombinant PAP in fish was determined. The experiment revealed that there was the high expression of PAP gene in blood and heart of carp fish |
