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โครงสร้างบางส่วนของยีน cyp1a ในปลากะพงขาว (Lates calcarifer) (The Partial Structure of cyp1a Gene of Sea Bass (Lates calcarifer)) |
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| รหัสดีโอไอ | |
| Creator | วราลี งามดี |
| Title | โครงสร้างบางส่วนของยีน cyp1a ในปลากะพงขาว (Lates calcarifer) (The Partial Structure of cyp1a Gene of Sea Bass (Lates calcarifer)) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 356 |
| Keyword | sea bass, cyp1a, Lates calcarifer |
| ISSN | 8578664 |
| Abstract | In this study, the DNA fragment of cyp1a (cytochrome P450, family 1, subfamily A) gene of sea bass (Lates calcarifer) was cloned and sequenced. DNA was isolated from liver and amplified by PCR (Polymerase Chain Reaction). The cyp1a amplicons, 649 bp in length were then inserted into pGEM ? -T Easy plasmid and transformed into Escherichia coli DH5? competent cells. The partial cyp1a gene sequences of sea bass showed highly similarity (95% identity (127/134, E value = 2e-50)) (retrieved on April 15, 2013) with sea perch (Lateolabrax japonicus) (GenBank accession no. GU373806). The cyp1a gene structure comprises 3 exons (134, 88 and 126 bp, respectively) and 2 introns (91 and 210 bp, respectively). This partial structure and sequences of cyp1a gene can apply to study the expression levels and used as a useful biomarker of Polycyclic Aromatic Hydrocarbons contaminated in the aquatic environment. |
