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การเปลี่ยนแปลงการแสดงออกของยีน epithelial-to-mesenchymal transition ในเซลล์มะเร็งท่อน้ำดีโดยโปรตีน periostin (Alteration of Epithelial-to-Mesenchymal Transition Gene Expression in Periostin-treated Cholangiocarcinoma) |
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| รหัสดีโอไอ | |
| Creator | สุชาดา ศิริตันหยง |
| Title | การเปลี่ยนแปลงการแสดงออกของยีน epithelial-to-mesenchymal transition ในเซลล์มะเร็งท่อน้ำดีโดยโปรตีน periostin (Alteration of Epithelial-to-Mesenchymal Transition Gene Expression in Periostin-treated Cholangiocarcinoma) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 392 |
| Keyword | epithelial-to-mesenchymal transition, periostin, cholangiocarcinoma |
| ISSN | 8578664 |
| Abstract | Periostin (PN) is increased in cholangiocarcinoma (CCA) tissues and relates to patient poor prognosis. Epithelial-to-mesenchymal transition (EMT) is a process characterized by changes of carcinoma to mesenchymal cell and essential for cancer progression. This study aims to investigate the expression of EMT-related genes in PN-induced CCA cell migration. Nine EMT genes including two matrix metalloproteinase genes (MMP-10, MMP-13), two mesenchymal genes (ASMA, VIM), two epithelial genes (CK19, CDH1), and three EMT-related transcription factors (SNAIL1, SLUG, TWIST2) were explored expression levels in cells with and without PN treatment by real time PCR. The results showed the up-regulated expressions (>1.2 fold) of MMP-10, SNAIL1, TWIST2, ASMA, and VIM, and significant up-regulation of MMP-13 whereas CK19 was significantly down-regulated (?0.8 fold) in PN-treated CCA cells. No alterations of SLUG and CDH1 were observed. The different results in PN- and transforming growth factor-?-treated CCA cells reveal specific EMT gene expression pattern depending on the stimulant. The understanding of EMT genes in PN-induced CCA cells helps to inhibit cancer progression. |
