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ระดับการแสดงออกของยีน transposase ในหอยเจดีย์ (Cerithidea cingulata) วัยอ่อนที่ได้รับสาร 17?-estradiol (E2) (Gene expression of transposase in juvenile horn snails (Cerithidea cingulata) exposed to 17?-estradiol) |
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| รหัสดีโอไอ | |
| Creator | ศรีวิกาญจน์ กรุมรัมย์ |
| Title | ระดับการแสดงออกของยีน transposase ในหอยเจดีย์ (Cerithidea cingulata) วัยอ่อนที่ได้รับสาร 17?-estradiol (E2) (Gene expression of transposase in juvenile horn snails (Cerithidea cingulata) exposed to 17?-estradiol) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | S.1 |
| Journal No. | S1 (Special Issue 1) |
| Page no. | 325-328 |
| Keyword | horn snail, Cerithidea cingulata, E2, semi-quantitative RT-PCR, transposase |
| ISSN | 8578664 |
| Abstract | Analysis of gene expression of transposase in juvenile horn snails (Cerithidea cingulata)exposed to 17?-estradiol (E2), the endrocrine disrupting chemicals (EDCs), by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) was investigated in this study. Horn snail tissue total RNAs were prepared and mRNA (messenger RNA) was subsequently converted into a complementary DNA (cDNA) using a retroviral reverse transcriptase. The horn snails exposed to E2by immersion at 10 ng/L compared to the control group, E2at 0 ng/L, for 8, 16 and 32 hours were examined. Semi-quantitative RT-PCR was analyzed using the relative expression ratio between transposase and the reference gene, 28S rRNA. The results showed that the relative expression levels of juvenile horn snails exposed to E2 at 10 ng/L for 32 hours were 0.23?0.03, higher than that of the control group (0.18?0.02), but no statistically significant difference (P>0.05). Therefore, the E2exposure time of the juvenile horn snails should be extended or use other sensitivity techniques. This could be a crucial factor for transposase and juvenile horn snails to be used as a sensitive biomarker of exposure for detecting E2contamination of the aquatic environments. |
