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การศึกษาปฏิสัมพันธ์ของโปรตีน prefoldin กับโปรตีนคัลมอดุลินและโปรตีนคล้าย คัลมอดุลินจากข้าว (Oryza sativa L.) |
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| รหัสดีโอไอ | |
| Creator | 1. จุฑามาศ อนันทยานนท์ 2. ธีรพงษ์ บัวบูชา |
| Title | การศึกษาปฏิสัมพันธ์ของโปรตีน prefoldin กับโปรตีนคัลมอดุลินและโปรตีนคล้าย คัลมอดุลินจากข้าว (Oryza sativa L.) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 6 |
| Journal No. | 1 |
| Page no. | 35 |
| Keyword | calcium sensor protein, calmodulin, rice, yeast two-hybrid system |
| ISSN | 8578664 |
| Abstract | Concentration of calcium, which is a second messenger, increases rapidly in cytosol when plants encounter environmental stress conditions. Calcium signals are then conveyed by the action of calcium sensors. Calmodulin (CaM), which is one of the calcium sensors, will bind to calcium ions and transmit the calcium signal by binding to and activating target proteins within the cell. The activities of target proteins affect physiological responses to specific stimuli received by plant cells. In previous study, we have identified several putative OsCaM1 target proteins from rice (Oryza sativa L.) by cDNA expression library screening. In this research, the yeast two-hybrid system was performed to examine the interaction of CaM and CML proteins with Prefoldin, which is one of the identified putative target proteins. The result showed that Prefoldin was found to bind OsCML4 both in the presence and in the absence of calcium but not with CaM. |
