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การโคลนและศึกษาคุณสมบัติของยีน OSB2 ซึ่งควบคุมการสังเคราะห์ แอนโทไซยานินในข้าว |
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| รหัสดีโอไอ | |
| Creator | 1. พูนศรี อินต๊ะ 2. วิศิษฎ์ พงศ์บุรพัฒน์ 3. แสงทอง พงษ์เจริญกิต 4. ศรีเมฆ ชาวโพงพาง 5. วราภรณ์ แสงทอง 6. ช่อทิพา สกูลสิงหาโรจน์ |
| Title | การโคลนและศึกษาคุณสมบัติของยีน OSB2 ซึ่งควบคุมการสังเคราะห์ แอนโทไซยานินในข้าว |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 6 |
| Journal No. | 1 |
| Page no. | 25 |
| Keyword | OSB2 gene, rice, anthocyanin |
| ISSN | 8578664 |
| Abstract | The OSB2 gene encodes myc transcription factor which regulates expression of several structural genes involved with anthocyanin biosynthesis in rice. In this study, the complete coding sequences (cds) of OSB2 genes were isolated from young leaves and developing seeds of rice by RT-PCR. Nucleotide sequencing analysis revealed that all 3 black rice varieties, Lerm-Poa, Hom-nin and Khum had a single open reading frame (ORF) of 1356 bp which were 99 % identical to the OSB2 gene reported in GenBank (accession no. AB021080). These rice varieties also had an OSB2 gene containing a single ORF of 1101 bp with 99 % identity with the 1356 bp fragments except for the deletion of the 2nd exon (255 bp deletion). The deduced amino acid sequences of the 1356 and the 1101 bp ORFs are composed of 451 and 366 amino acid residues, respectively, which showed 99 % similarity to those of OSB2 (accession no. BAB64302). The results suggested that the OSB2 genes from this study may have 2 forms. Alternatively, the 1356 bp and 1101 bp ORFs may be due to alternative splicing of mRNA transcribed from a single OSB2 gene in rice genomes of these black rice varieties. The cloned OSB2 genes will be further analyzed for structure and gene functions to understand the regulation of anthocyanin biosynthesis in colored rice. |
