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Alteration of epithelial-to-mesenchymal transition gene expression in periostin-treated cholangiocarcinoma |
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| รหัสดีโอไอ | |
| Creator | 1. Suchada Siritunyong 2. Jumaporn Sonongbua 3. Peti Thuwajit 4. Chanitra Thuwajit |
| Title | Alteration of epithelial-to-mesenchymal transition gene expression in periostin-treated cholangiocarcinoma |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2556 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 6 |
| Journal No. | 1 |
| Page no. | 77 |
| Keyword | epithelial-to-mesenchymal transition, periostin, cholangiocarcinoma |
| ISSN | 8578664 |
| Abstract | Periostin (PN) is increased in cholangiocarcinoma (CCA) tissues and relates to patient poor prognosis. Epithelial-to-mesenchymal transition (EMT) is a process characterized by changes of carcinoma to mesenchymal cell and essential for cancer progression. This study aims to investigate the expression of EMT-related genes in PN-induced CCA cell migration. Nine EMT genes including two matrix metalloproteinase genes (MMP-10, MMP-13), two mesenchymal genes (ASMA, VIM), two epithelial genes (CK19, CDH1), and three EMT-related transcription factors (SNAIL1, SLUG, TWIST2) were explored in terms of their expression levels in cells with and without PN treatment by real time PCR. The results showed up-regulations (>1.2 fold) of MMP-10, SNAIL1, TWIST2, ASMA, and VIM, and a significant up-regulation of MMP-13 whereas CK19 was significantly down-regulated (?0.8 fold) in PN-treated CCA cells. No alterations of SLUG and CDH1 were observed. The different results in PN- and transforming growth factor-?-treated CCA cells reveal specific EMT gene expression pattern depending on the stimulant. Understanding of EMT genes in PN-induced CCA cells might help in inhibiting cancer progression. |
