|
Nuclear migration behavior of Coprinopsis cinerea, during the monokaryotic mating and the relationship between the clamp cell number and fruiting body formation |
|---|---|
| รหัสดีโอไอ | |
| Creator | 1. Prayook Srivilai 2. Ananyaphon Prommetta 3. Panida Loutchanwoot |
| Title | Nuclear migration behavior of Coprinopsis cinerea, during the monokaryotic mating and the relationship between the clamp cell number and fruiting body formation |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2555 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 5 |
| Journal No. | 1 |
| Page no. | 34 |
| Keyword | Coprinopsis cinerea, nuclear migration, clamp cell, DAPI, fruiting body formation |
| ISSN | 8578664 |
| Abstract | Coprinopsis cinerea is a model mushroom ประยุกต์ ศรีวิไล และคณะ Thai J. Genet. 2011, 5(1) : 34-49 35 used for studying the growth, development, fruiting body formation and genetic systems of the basidiomycetous mushrooms. This research aimed to study the nuclear migration behavior during the monokaryotic mating of 9 strains of C. cinerea namely PS001, PS002, PS001-1, PS002-1, 218, AT8, Uv-6031, MK54 and Okayama7. The results revealed that there are three patterns of nuclear migration, which are nuclear donor, nuclear recipient and both nuclear donor and recipient. The behavior of nuclear donor or recipient of each C. cinerea strain varied depending upon the mating partners, as the B mating type gene exerted clear effects on nuclear migration. During the monokaryotic mating, the positions of nuclei in the hypha were located by staining with the fluorogenic dye 4?,6-Diamidino-2-phenyl indole dihydrochloride (DAPI). The results showed that the nuclear positions within the hypha varied in each C. cinerea strain. Moreover, at least two types of hyphal fusion were observed, the hyphal tip fusion and lateral fusion. This study also found that the numbers of clamp cells did not correlate with the numbers of fruiting bodies. In addition, the dikaryons obtained from the monokaryotic matings resulted in a higher number of fruiting bodies than those of the homokaryon AmutBmut. |
