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Predominant genotypes and alleles of the Val-9Ala polymorphisms in the mitochondrial targeting sequence leading the manganese superoxide dismutase gene associated with their superoxide dismutase activity and total antioxidant status of healthy Thai subjec |
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| รหัสดีโอไอ | |
| Creator | 1. Watcharee Attatippaholkun 2. Kornwipa Wikainapakul 3. Saranatra Waikakul 4. Sirirat Tunsakul |
| Title | Predominant genotypes and alleles of the Val-9Ala polymorphisms in the mitochondrial targeting sequence leading the manganese superoxide dismutase gene associated with their superoxide dismutase activity and total antioxidant status of healthy Thai subjects |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2555 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 5 |
| Journal No. | 1 |
| Page no. | 89 |
| Keyword | manganese superoxide dismutase (MnSOD), mitochondrial targeting sequence (MTS), Val-9Ala polymorphism, superoxide dismutase (SOD), total antioxidant status (TAS), healthy Thai subjects |
| ISSN | 8578664 |
| Abstract | Genotype distributions and allele frequencies of the Val-9Ala polymorphisms in the mitochondrial targeting sequence leading the manganese superoxide dismutase gene as well as associations with their superoxide dismutase activity and total antioxidant status of healthy Thai subjects were determined. The Val-9Ala polymorphisms in MTS-MnSOD gene of 221 healthy Thais including 135 females and 86 males were genotyped by real-time PCR. The SOD activity of whole blood cells and plasma total antioxidant status (TAS) of each subject were assayed. The Val/Val and the Val/Ala genotypes were found to be predominant rather than the Ala/Ala genotype. The Val allele frequencies in healthy Thai subjects, both females and males, were similar to those previously reported in healthy Chinese, Japanese and Korean subjects. The average values of SOD activity and TAS level in healthy Thais were 2,129.?778 U/g Hb and 1.27?0.26 mmol/L, respectively. The association of each genotype with SOD activity as well as TAS levels showed that the average values of SOD activity and TAS in healthy Thais with the Val/Val and the Val/Ala genotypes were not statistically different (p>0.05) and not lower than those of the Ala/Ala genotypes. In conclusion, the predominant genotypes were the Val/Val and the Val/Ala and they were not associated with low levels of SOD and TAS. |
