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Low genetic variation of Boesenbergia tenuispicata, a species endangered and endemic to Thailand, using RAPD markers |
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| รหัสดีโอไอ | |
| Creator | Ongkarn Vanijajiva |
| Title | Low genetic variation of Boesenbergia tenuispicata, a species endangered and endemic to Thailand, using RAPD markers |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2555 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 5 |
| Journal No. | 1 |
| Page no. | 67-78 |
| Keyword | Boesenbergia tenuispicata, RAPD marker, genetic variation |
| ISSN | 8578664 |
| Abstract | Random amplified polymorphic DNA (RAPD) marker was used to analyze genetic structure of the endemic Thai plant Boesenbergia tenuispicata K. Larsen. Twenty samples from each population were collected during January 2011 January 2012 in Krabi, Thailand. Genomic DNA was extracted from fresh leaves. Nineteen RAPD primers were initially screened and eight primers (OPA-18, OPAM-01, OPAM-03, OPAM-12, OPB-14, OPD-03, OPD-18 and OPZ-03) were chosen for further analysis. A total of 72 DNA fragments, varying from 100-2,800 bp, were amplified, of which 12 (16.66%) were polymorphic. A dendrogram was constructed based on polymorphic bands using the SPSS program (version 18). Three clusters could be separated with similarity coefficients ranging from 0.840-1.000. Therefore, RAPD marker is a rapid and suitable method for analysis of genetic diversity among endemic B. tenuispicata samples. Nevertheless, low polymorphic bands were detected, indicating that the genetic diversity of B. tenuispicata was low, and all populations most likely consisted of the same genotype. The result suggested that the management for the conservation of genetic variability in B. tenuispicata should aim to preserve every population. |
