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Next generation sequencing (NGS) technologies and their applications in omics-research |
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| รหัสดีโอไอ | |
| Creator | 1. Alisa Wilantho 2. Oranud Praditsup 3. Wanwisa Charoenchim 4. Supasak Kulawonganunchai 5. Anunchai Assawamakin 6. Sissades Tongsima |
| Title | Next generation sequencing (NGS) technologies and their applications in omics-research |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2555 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 5 |
| Journal No. | 2 |
| Page no. | 104-129 |
| Keyword | NGS technology, reads, mapping tools |
| ISSN | 8578664 |
| Abstract | The main objective of Next generation sequencing (NGS) technology is to quickly and efficiently read the underlying sequence of an organism by means of massively parallel sequencing. Comparing with Sanger's technology, NGS can produce much larger number of sequence reads that cover huge number of bases per run. Three major platforms of NGS technologies are currently in used, namely 454/Roche, Solexa/Illumina and SOLiD/Life Technology (ABI). These platforms differ in sequencing principles, sequence read formats and number of read outputs. Moreover, these platforms are useful in the different applications in several omic-research domains including de novo sequencing, target resequencing, RNA sequencing and metagenomics etc. This review article presents the overview of the three major NGS technologies. We cover the principle of sequencing in three platforms (454/Roche, Solexa/Illumina and SOLiD/ABI), applications, and mapping tools used to discover the variation of read sequences leading to deciphering the problems in the other research topics |
