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Microsatellite-based analysis of genetic variation in hatchery populations of Asian seabass, Lates calcarifer (BLOCH, 1790) |
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| รหัสดีโอไอ | |
| Creator | Panom Krachangpoj Sodsuk |
| Title | Microsatellite-based analysis of genetic variation in hatchery populations of Asian seabass, Lates calcarifer (BLOCH, 1790) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2555 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 5 |
| Journal No. | 2 |
| Page no. | 166-182 |
| Keyword | Asian seabass, Lates calcarifer, genetic variation, microsatellite |
| ISSN | 8578664 |
| Abstract | Genetic variation based on microsatellite markers was comparatively analysed among 5 hatchery populations of Asian seabass, Lates calcarifer (BLOCH, 1790). Four populations were from the broodstocks of Krabi, Trang, Nakhon Si Thammarat and Rayong Coastal Fisheries Research and Development Center, and one population is the progeny. From the 12 microsatellite loci screened, LcaM27F (AF404089) was found to be the most polymorphic locus with 13 variant alleles, whereas LcaM06 (AF404075) was found to be the only monomorphic. Genetic variabilities per locus including averages number of alleles (Na), effective number of alleles (Ae), allelic richness (R), observed heterozygosity (Ho) and expected heterozygousity (He) were Na = 4.00004.5000, Ae = 2.55673.0091, R = 3.45103.8841, Ho = 0.56270.6278 and He = 0.53910.6092, respectively. When all loci were analysed, the population from Rayong and the progeny were found to deviate from Hardy-Weinberg equilibrium (PHWE>0.05). However, the levels of genetic variation observed in all 5 populations were similar, and were lower than the level of wild and hatchery populations in Thailand reported by previous studies. Analyses of genetic structure and differences among populations showed that the 5 populations can be genetically classified as a single group, with genetic differences between populations within the group. Results from the analyses of each pair of populations together with each populations' pedigree revealed differences of the populations in details and reasons leading to their changes in their genetic composition. Due to low genetic diversity level in the 5 hatchery populations of Asian seabass compared to that of wild populations, a recommendation is that founding populations of Asian seabass broodstock should have higher level of genetic diversity to obtain good quality and high potential broodstock for Thai aquaculture. |
