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Study of genetic diversity of Thai native buffaloes using mitochondrial DNA marker |
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| รหัสดีโอไอ | |
| Creator | 1. Kamonpun Chauckwon 2. Monchai Duangjinda 3. Yupin Phasuk 4. Virote Pattarajinda 5. Sarayut Wonnasuit 6. Kitti Koobkaew |
| Title | Study of genetic diversity of Thai native buffaloes using mitochondrial DNA marker |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2553 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 3 |
| Journal No. | 2 |
| Page no. | 149 |
| Keyword | genetic diversity, Thai native buffalo,mitochondrial DNA |
| ISSN | 8578664 |
| Abstract | The aim of this study was to study diversityand genetic classification of Thai native buffaloesusing mitochondrial DNA marker. From 165 buffaloesof 6 different habitats of Thailand represented byChaingrai (C, n=30), Khon Kaen (K, n=39), Surin(S, n=29), Uthaithani (U, n=26), Nakhon Si Thammarat(N, n=17) and Trang province (T, n=24), 300 bpfragments of mtDNA (D-loop) were successfullyamplified. Analysis of mtDNA (D-loop) sequencesrevealed 7 different haplotypes resulting from 32polymorphic sites and one insertion-deletion. Thisresult showed that Thai native buffaloes haveflourishing of genetic diversity. The phylogenetictree analysis from Nei?s genetic distance andcharacteristics of habitat revealed that Thai nativebuffaloes could be classified into 3 groups: (1)mountain buffalo: the Chiangrai native buffaloes, (2)ocean buffalo: the Trang native buffaloes, and (3)river basin buffalo: the Khon Kaen, Surin, Uthaithani,Nakhon Si Thammarat native buffaloes. The analysisof genetic structure showed moderate geneticdifferentiation between six groups (Gst = 0.257). Theinformation generated by this study will greatly aidin the establishment of effective breeding programsand conservation genetics of Thai native buffaloes. |
