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Microsatellites for parentage analysis in an oil palm breeding population. |
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| รหัสดีโอไอ | |
| Creator | 1. Songkran Thongthawee 2. Palat Tittinutchanon 3. Hugo Volkaert |
| Title | Microsatellites for parentage analysis in an oil palm breeding population. |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2553 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 3 |
| Journal No. | 2 |
| Page no. | 172 |
| Keyword | microsatellites, oil palm, DNAfingerprinting, paternity, parentage |
| ISSN | 8578664 |
| Abstract | Oil palm is an important oil crop in tropicalregions. Breeding for improved varieties yieldingmore oil per area planted is a slow and difficultprocess. Establishing control-pollinated oil palmprogenies for breeding purposes is not withoutproblems as it takes a long time and many steps frompollination, seed collection, germination, to fieldplanting. At each of these steps errors could arise.A breeding programme requires 100% certainty ofcorrect parentage in the progeny trials. The allelefrequencies at eight oil palm microsatellite loci weredetermined among the parents and progenies in anoil palm breeding programme. Paternity and/orparentage likelihoods were calculated for the offspring.Even though the oil palm breeding populations inSoutheast Asia are assumed to have low geneticdiversity because of their recent introduction fromjust a few founder trees, the eight loci combined hadsufficient power to detect errors with great confidence.Three clear planting errors and one presumedpollination error were detected among 245 individualsin 6 full sib families. The overall genetic structureof the oil palm breeding population was evaluatedusing the same loci. A combination of four loci wasalready sufficient to reach a non-exclusion levelbelow 1% for the detection of planting errors. Todetect pollination errors confidently, 7 or 8 lociwould be necessary. |
