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Detection of Contaminated Bacteria in Frozen Minced Pork Using Multiplex Polymerase Chain Reaction Technique |
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| รหัสดีโอไอ | |
| Creator | 1. Narumol Thanananta 2. Theerachai Thanananta |
| Title | Detection of Contaminated Bacteria in Frozen Minced Pork Using Multiplex Polymerase Chain Reaction Technique |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2552 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 2 |
| Journal No. | 2 |
| Page no. | 125 |
| Keyword | multiplex PCR, bacterial detection, food-born pathogenic bacteria |
| ISSN | 8578664 |
| Abstract | Multiplex polymerase chain reaction (mPCR) technique without DNA extraction was developed for detection of pathogenic bacteria in frozen minced pork. Five primer pairs were designed for amplification of target DNA fragments of 5 bacterial species namely Campylobacter fetus, Escherichia coli, Listeria monocytogenes, Salmonella enterica serovar Typhi and Vibrio cholerae. The results showed that the new method of multiplex PCR technique in this study was specific to the target bacteria with sensitivity at 104 cells/g |
