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Development of microsatellite markers for Jatropha curcas L. |
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| รหัสดีโอไอ | |
| Creator | 1. Yatavee Rattanamanee 2. Surin Peyachoknagul, Nitsri Sangduen 3. Vipa Hongtrakul |
| Title | Development of microsatellite markers for Jatropha curcas L. |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2552 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 2 |
| Journal No. | 2 |
| Page no. | 145-154 |
| Keyword | microsatellite markers, Jatropha curcas L. |
| ISSN | 8578664 |
| Abstract | The objective of this research is to develop microsatellite DNA markers for classification and species specific identification of physic nut (Jatropha curcas L.). DNAs from physic nut (SS20-Sbr3, USA, Nakhon Ratchasima) were digested with MseI restriction enzyme, then ligated to adapters. The products were PCR amplified before hybridization to biotin-oligonucleotide probes of B-(GA)15, B-(CA)15, B-(ACC)10 and B-(CCT)10 containing magnetic beads. The DNA fragments were then PCR amplified again before ligation to plasmid vector. The recombinant plasmids were heat-shock transformed to supercompetent E. coli |
