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Identification of sugar apple cultivars Nua and Nang using AFLP and SCAR markers |
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| รหัสดีโอไอ | |
| Creator | 1. Narumol Thanananta 2. Theerachai Thanananta |
| Title | Identification of sugar apple cultivars Nua and Nang using AFLP and SCAR markers |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2551 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 1 |
| Journal No. | 2 |
| Page no. | 122 |
| Keyword | Sugar apple, Annona squamosa L., AFLP, SCAR |
| ISSN | 8578664 |
| Abstract | Amplified fragment length polymorphism (AFLP) technique was used to identify sugar apple (Annonasquamosa L.) cultivars Nua and Nang. The total of 64 EcoRI-MseI primer-pair combinations were screenedand all of them could produce DNA fingerprinting. Eight AFLP markers were clearly polymorphic amongthe 2 cultivars which were capable to distinguish the sugar apple varieties between Nua and Nang. Fourpolymorphic AFLP fragments were subsequently cloned and sequenced for sequence characterized amplified region (SCAR) primer design. The primers were tested with individual DNA samples and the result showedthe polymorphisms that were identified from the SCAR markers with specific to only sugar apple variety Nua. |
