|
Enzyme Production of Trichoderma spp. Isolated from Soil and Mushroom Spawn |
|---|---|
| รหัสดีโอไอ | |
| Creator | Payungsak Rauyaree |
| Title | Enzyme Production of Trichoderma spp. Isolated from Soil and Mushroom Spawn |
| Contributor | Tassanaporn Tadsakorn |
| Publisher | Department of Agriculture |
| Publication Year | 2567 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 42 |
| Journal No. | 1 |
| Page no. | 62-70 |
| Keyword | Trichoderma spp, cellulase, pectinase, amylase |
| URL Website | tci-thaijo.org/index.php/thaiagriculturalresearch |
| Website title | Thai Agricultural Research Journal |
| ISSN | 3027-7272 |
| Abstract | Twenty-nine isolates of Trichoderma spp. obtained from soil and mushroom spawn samples during 2019 to 2021 were used to evaluate their degrading-enzymes synthesis efficiency on selective media. Degrading-enzymes synthesis efficiency was done by zone clearing technique and by calculating hydrolysis capacity (HC) value. The results revealed that isolates T-1, T-14 and T-22 showed the highest efficiency to synthesize pectinase, cellulase and amylase enzymes at HC values of 7.73, 7.13 and 5.00 respectively. Isolates T-1, T-14 and T-22 were identified by ITS4 and ITS5 gene sequencing. The results showed that T-1, T-14 and T-22 were Trichoderma asperellum (Samuels) Lieckf. & Nirenberg with 100, 99 and 99% identity. T-1 and T-14 were isolated from soil in Photharam, Ratchaburi province and Dan Makham Tia, Kanchanaburi Province respectively. Whereas, T-22 was isolated from mushroom spawn in Damnoen Saduak, Ratchaburi Province. |
