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Enhancement of a Co-PCR Technology for Detection of Bacterial Fruit Blotch Pathogen, Acidovorax citrulli, in Watermelon Seed |
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| รหัสดีโอไอ | |
| Creator | Supananya Chansri |
| Title | Enhancement of a Co-PCR Technology for Detection of Bacterial Fruit Blotch Pathogen, Acidovorax citrulli, in Watermelon Seed |
| Contributor | Kanchanaphorn Sawangchaitham, Thanyaluk Thaitae, Jutatape Watcharachaiyakup |
| Publisher | Department of Agriculture |
| Publication Year | 2566 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 41 |
| Journal No. | 1 |
| Page no. | 14-27 |
| Keyword | seed certification, watermelon, cucurbit, bacterial fruit blotch |
| URL Website | https://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | Enhancement of Co-PCR technology for the detection of Acidovorax citrulli (Ac) using the 521 primer set was developed in this study. The four primers comprised AC-ORF12F and AC-ORF1F4 at 0.4 ?M, and primers AC-ORF13R and AC-ORF3F3 at 0.2 ?M with the specificity of annealing temperature at 72 o C were employed. Results revealed that detection limit of the target DNA detection was 1 pg. The detection limit of this method in the 0.01% contamination seed lot was 37 colony forming unit (CFU). By comparison, the standard ISHI-Veg protocol was unable to detect Ac at the same level of contamination. |
