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The Classification of Allium cepa L. by Flow Cytometric Estimation of DNA Content in the Innermost Bulb |
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| รหัสดีโอไอ | |
| Creator | Pumipat Tongyoo |
| Title | The Classification of Allium cepa L. by Flow Cytometric Estimation of DNA Content in the Innermost Bulb |
| Contributor | Bangon Chaiyata, Thananya Thongsanit, Punyavee Dechkrong, Julapark Chungwongse |
| Publisher | Department of Agriculture |
| Publication Year | 2566 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 41 |
| Journal No. | 2 |
| Page no. | 193-202 |
| Keyword | flow cytometry, nuclear DNA, shallot, onion |
| URL Website | https://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | The genus Allium contains more than 920 species. Some species of Allium cannot be identified morphologically. The aim of this project is to assay the DNA content of nuclei of onion, shallot, Indian onion, and potato onion by flow cytometer. The first experiment involved the assay of DNA content of each layer of the onions' scales. Results showed that the ploidy level was different in each layer of the scales. While most of the innermost scales were diploid, the outer layers contained more of the polyploid nuclei; the farther from the center of the scales, the lesser the diploid nuclei were found. Therefore, in the second experiment, DNA content from the innermost scales of each Allium was investigated. The results clearly differentiated the Allium samples into two groups. The first group included onion and potato onion from Myittha with an average DNA content between 33.53-33.7 pg.2C-1. While the second group included potato onions from Monywa and from Myingyan, shallot (Kanchanaburi), shallot (Lamphun), and Indian onion with an average DNA content between 31.54-32.40 pg.2C-1 |
