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Characterization of Xanthomonas causing of Bacterial Leaf Spot of Tomato and Pepper in Thailand |
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| รหัสดีโอไอ | |
| Creator | Santipong Sitthitanasin |
| Title | Characterization of Xanthomonas causing of Bacterial Leaf Spot of Tomato and Pepper in Thailand |
| Contributor | Chanyanut Korakngam, Tippawan Kanhayart, Nuttima Kositcharoenkul, Sujin Patarapuwadol, Wichai Kositratana, Jutatape Watcharachaiyakup |
| Publisher | Department of Agriculture |
| Publication Year | 2563 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 38 |
| Journal No. | 1 |
| Page no. | 80-89 |
| Keyword | Xanthomonas, bacterial leaf spot disease, tomato, pepper, multilocus sequence, housekeeping genes |
| URL Website | http://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | Bacterial leaf spot is an important disease of tomato and pepper, and is a threat to seed industry in particular. Lately, Xanthomonas species causing bacterial leaf spot disease of tomato and pepper had been re-classified, using molecular techniques, into four species,namely X. euvesicatoria, X. gardneri, X. perforans, and X. vesicatoria. In this study wecharacterized bacterial pathogens of leaf spot disease of tomato and pepper in Thailand.A total of 38 isolates of disease causing bacteria were used in this study, 24 of which wereobtained from Department of Agriculture's Culture Collection Center. They were collectedduring 1987-2018 and already identified as X. vesicatoria. The other 14 isolates wecollected from the fields in Khon Kaen and Chaiyaphum provinces in 2018. These bacterialisolates were identified as Xanthomonas sp. based on morphology, physiological andbiochemical properties. The selected 18 isolates were further identified by using multilocussequence analysis (MLSA) of gyrB, efp, dnaK and atpD genes and specific primer set ofeach species. As a result, two species were identified. Ten isolates from pepper wereidentified as X. euvesicatoria, and the other 7 from 8 isolates from tomato were X. perforans.However, PCR amplification from Bs-XpF/ Bs-XpR primers specific to X. perforans wasno found. None of the isolates in this study were X. gardneri or X. vesicatoria. |
