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Development and Method Validation of Ochratoxin A Analysis in Cereals using High Performance Liquid Chromatography - High Resolution Mass Spectrometry |
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| รหัสดีโอไอ | |
| Creator | Thitaporn Rutrin Tongngamdee |
| Title | Development and Method Validation of Ochratoxin A Analysis in Cereals using High Performance Liquid Chromatography - High Resolution Mass Spectrometry |
| Contributor | Adisorn Jettanajit , Ratchanee Ragsattayanan , Kanokwan Ponchim , Taweesak Tongngamdee |
| Publisher | Department of Agriculture |
| Publication Year | 2563 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 38 |
| Journal No. | 2 |
| Page no. | 116-127 |
| Keyword | cereal, HPLC-HRMS, method validation, Ochratoxin A, wheat |
| URL Website | https://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | The objective of this study was to develop an analytical method for ochratoxinA in cereals, modified from AOAC official method 2000.03 using high performance liquidchromatography - high resolution mass spectrometry (HPLC-HRMS). The ion polarity(negative and positive mode) and the different ratios between 0.1% formic acid in water(v/v) and acetonitrile in mobile phase at 20:80, 30:70 and 40:60 were studied. We found thatoptimal conditions for ochratoxin A analysis using HPLC-HRMS were negative mode for ionpolarity and 0.1% formic acid in water acetonitrile ratio of 30: 70 as HPLC mobile phase.The method was then validated to ensure that it would deliver reliable results. The validationresults demonstrated that the working range, limit of detection and limit of quantitation were0.50 20 ? g/kg, 0.20 ? g/kg and 0.50 ? g/kg, respectively. Moreover, the accuracy andprecision of the method are within acceptance criteria. (%Recovery 72.54 - 116.60, %RSD13.10 - 13.69). All results demonstrated that the developed method could detect the presenceof ochratoxin A in cereals at the level below permitted levels of the importing countries'regulation. The method is accredited ISO/IEC 17025 by Bureau of Laboratory Standards,Department of Medical Sciences. and is being used as a standard method for ochratoxinA analysis in cereal at laboratory of Plant Standard and Certification Division, Departmentof Agriculture. |
