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Detection of Acidovorax citrulli by Co-operational Polymerase Chain Reaction for Seed Health Testing of Watermelon Seed |
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| รหัสดีโอไอ | |
| Creator | 1. Chanyanut Korakngam 2. Jutatape Watcharachaiyakup 3. Sujin Patarapuwadol 4. Wichai Kositratana |
| Title | Detection of Acidovorax citrulli by Co-operational Polymerase Chain Reaction for Seed Health Testing of Watermelon Seed |
| Publisher | Department of Agriculture |
| Publication Year | 2561 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 36 |
| Journal No. | 3 |
| Page no. | 279-292 |
| Keyword | โรคผลเน่าในแตงโม, Acidovorax citrulli, bacterial fruit blotch, Co-PCR, seed detection |
| URL Website | http://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | The bacterial fruit blotch (BFB) caused by Acidovorax citrulli is a serious pathogenfor cucurbits seed production. This bacterium is a seed-borne and seed-transmittedpathogen with long-term survival in seeds. Contaminated seeds are an important source ofprimary inoculum for the spreading of BFB disease in seed beds. Detection technique forthe pathogen which is reliable, effective, sensitive and specific is an important tool forcontrolling seed quality in both local and international seed trade. The Co-PCR was shownto be effective in detecting A. citrulli in the artificially inoculated watermelon seeds by seedsoaking. The samples were prepared by filtering the seed soaking liquid using bacterialfiltration system then extracted the bacterium DNA from the filtered remain and used asthe target DNA. Results indicated that this procedure could detect A. citrulli presented atthe level of 103 CFU/seed in an artificially inoculated watermelon seed stocks which had0.05% and 0.01% infestation rates. It could also detect the bacterium in naturally infectedwatermelon seeds. |
