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Seed Transmission of Columnea latent viroid in cucumber |
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| รหัสดีโอไอ | |
| Creator | 1. Kanokmanee Tansuwan 2. Kanungnit Reanwarakorn |
| Title | Seed Transmission of Columnea latent viroid in cucumber |
| Publisher | Department of Agriculture |
| Publication Year | 2561 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 36 |
| Journal No. | 2 |
| Page no. | 130-140 |
| Keyword | viroid, cucurbit, seed transmission, ไวรอยด์, พืชตระกูลแตง, การถ่ายทอดทางเมล็ด |
| URL Website | http://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | Columnea latent viroid (CLVd) seed transmission was studied in 6 cucumber varietiesby inoculating CLVd on 5 seedlings of each variety. Inoculated plants were confirmed theCLVd-infection by Reverse transcription ? polymerase chain reaction (RT ? PCR) technique.Cucurbit seeds were harvested from 30-35 days mature fruits after cross pollination of theinfected plants. The cucurbit seeds were determined for the presence of 370 bp DNAfragment of CLVd to confirm seed transmission. The results showed that 7 samples of3 varieties were 0.19 ? 1.77% seed transmission. All PCR products of the CLVd positivesamples were sequenced and aligned with GenBank database for sequence homology. Theywere 367-369 nucleotides in size and 97-99% sequence similarity with CLVd isolates. Theresults indicate that CLVd was seed transmission in cucumber plants. |
