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Efficacy of Trichoderma harzianum on Inhibition of the Causal Agent of Pummelo Tan Spot Disease (Phyllosticta citriasiana) |
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| รหัสดีโอไอ | |
| Creator | 1. Suthamas Na Nan 2. Patipat Jaipin 3. Sanong Jarintorn 4. Boonpiyathida Klongkaew |
| Title | Efficacy of Trichoderma harzianum on Inhibition of the Causal Agent of Pummelo Tan Spot Disease (Phyllosticta citriasiana) |
| Contributor | Department of Agriculture |
| Publisher | Department of Agriculture |
| Publication Year | 2560 |
| Journal Title | THAI AGRICULTURAL RESEARCH JOURNAL |
| Journal Vol. | 35 |
| Journal No. | 3 |
| Page no. | 321-287 |
| Keyword | Trichoderma, tan spot disease, pummelo |
| URL Website | http://at.doa.go.th/journal |
| Website title | THAI AGRICULTURAL RESEARCH JOURNAL |
| ISSN | 1258389 |
| Abstract | The efficacy of Trichoderma harzianumon for inhibition of Phyllosticta citriasiana,a causal agent of pummelo tan spot disease, was examined under laboratory condition atChiangrai Horticulture research center during 2014-2015. Forty four isolates of T.harzianum were obtained from soil samples collected from pummelo orchards atWiangkaen district, Chiangrai province and Chiangdao district, Chiangmai province using soildilution spread plate method. Three pure culture of P. citriasiana; CR-L01, CR-F09, andCM-F05 were isolated from tan spot symptom on leaf and fruit of pomelo using tissuetransplanting method. The mycelia growth of P. citriasiana isolate CR-F09 was cultured oneight types of agar media based on CRD criterion with five replications for each treatment.OMA, PoDA, and PSA were effective media to cultivate P. citriasiana as they provided thehighly percentage of mycelium growth with 98.2, 96.4 and 88.9%, respectively after 14-dayof incubation. The inhibition efficacy of 17 isolates of T. harzianum on P. citriasiana wasdetermined by dual culture method in vitro. After 3-day incubation, T. harzianum isolatesT4, T9, T10, T14, T21, T29, and T35 presented the mycelium growth inhibition at 43.3,50.0, 50.0, 46.7, 43.3, 43.3, and 50.0 %, respectively. Strain of T35 and T10 showed highlypercentage mycelium growth inhibition after 5-day of incubation with 52.1 and 51.0 %.The efficacy of T. harzianum isolates T35, commercial product of Trichoderma andazoxystrobin 25% W/V SC to control pummelo tan spot disease were also determinedunder field condition based on RCBD criterion with four replications for each treatment.The results showed that application of azoxystrobin at 10 ml/20 L of water was the mosteffective method to control pummelo tan spot disease compared to T. harzianumisolates T35 and commercial product of Trichoderma. |
