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Effect of Storage Temperature on Seed Viability and In Vitro Germination of Nobile Dendrobium Hybrids |
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| รหัสดีโอไอ | |
| Creator | 1. Waraporn Udomdee 2. Pei-Jung Wen 3. Shih-Wen Chin 4. Fure-Chyi Chen |
| Title | Effect of Storage Temperature on Seed Viability and In Vitro Germination of Nobile Dendrobium Hybrids |
| Publisher | Department of Agriculture |
| Publication Year | 2557 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 32 |
| Journal No. | 2 |
| Page no. | 201-207 |
| Keyword | nobile Dendrobium, TTC staining, seed storage, low temperature |
| URL Website | http://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | Nowadays, seed storage plays an important role for long-term conservation forplant genetic resources including orchids. Besides, asymbiotic germination provides anessential tool for commercial propagation and conservation purpose. Nobile-typeDendrobium hybrids are in high demand as decorative pot plants due in part to theirshowy flowers. In this study the effect of storage temperatures on seed viability andsubsequent germination capability of nobile Dendrobium hybrids was evaluated. Matureseeds from ripe capsules were collected from three cross combinations of nobileDendrobium hybrids; D. Spring Dream ?Apollon? x D. Hamana Lake ?Dream?, D. GoldenTower x D. 012-3, and D. 184-1 x D. 140-1 and air-drieD. The dried seeds were stored ateither room temperature (25+2๘ ?C) or -20 ?๘C for twenty four months. In vitro seedgermination and viability test by TTC stain were investigated. The germinability andviability varied on storage temperature as well as hybrid combinations and duration ofseed storage. The viability of seed stored at -20๘ ?C was higher than at room temperatureas revealed by their germination percentage. Stainability percentage of seed stored atroom temperature was severely low after 9 months of storage, while seed stored at -20๘Ccould maintain viability longer than at room temperature. Seed germination decreasedrapidly after 6 months at room temperature, whereas seeds stored at -20๘ ?C thegermination decreased after 20 months. Also, the seeds rarely developed to advanceseedling stages (stage 5 and 6) after 20 months of storage at -20 ?๘C. |
