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Diagnostic methods for the detection of pospiviroids in Solanaceous plants and seeds |
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| รหัสดีโอไอ | |
| Creator | Sukhontip Sombat |
| Title | Diagnostic methods for the detection of pospiviroids in Solanaceous plants and seeds |
| Publisher | Department of Agriculture |
| Publication Year | 2557 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 32 |
| Journal No. | 2 |
| Page no. | 164-177 |
| Keyword | เชื้อพอสพิไวรอยด์, พืชวงศ์ Solanaceae, เชื้อไวรอยด์, Potato spindletuber viroid detection, Pospiviroids, Solanaceae, Potato spindle tuber viroid |
| URL Website | http://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | In many countries phytosanitary regulations were applied to pospiviroid, Potatospindle tuber viroid (PSTVd) because it can cause economically important diseases onSolanaceous plants and seeds. Consequently, there is a need for a reliable and costeffectivegeneric testing method. PSTVd infected tomato seeds from North Carolina, USAwere used for evaluation the PSTVd detection method in U.S. vegetable laboratory, SouthCarolina. Total seed RNA was prepared using Trizol reagent and SDS- potassium acetatemethods. PSTVd can be detected by RT-PCR and Real time RT-PCR from single infectedseed. Real time RT-PCR was highly sensitive and effective. It can be detected frommixture of a single infected seed and healthy seeds in various ratios of tomato andSolanaceous seeds with two extraction methods. An initial investigation of PSTVd intomato breeder stock seed srevealed the presence of the viroid at greenhouse in theNorth Carolina area. PSTVd infection in solanaceous plants that were inoculated with sapfrom tomato infected seeds displayed severe symptoms in tomato plants, very mildsymptoms in pepper plants and symptomless in petunias plants at eight weeks afterinoculation. |
