|
In Vitro Culture Young Leafs and Shoot Tips of Wrightia religiosa Benth |
|---|---|
| รหัสดีโอไอ | |
| Creator | 1. Phopgao Buddharak 2. Warut U-kong |
| Title | In Vitro Culture Young Leafs and Shoot Tips of Wrightia religiosa Benth |
| Publisher | Department of Agriculture |
| Publication Year | 2554 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 29 |
| Journal No. | 3 |
| Page no. | 275-282 |
| Keyword | การเพาะเลี้ยงเนื้อเยื่อ, โมกพวง, สารควบคุมการเจริญเติบโตของพืช |
| URL Website | http://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | Wrightia religiosa is endemic species found only in Thailand, which is a rare and endangered species. A study of tissue culture is another way to increase the number. The young leaf of Wrightia religiosa. was cultured on Murashige and Skoog (MS) supplemented with various concentrations of thidiazuron (TDZ) namely 0.0, 2.0, 3.0 and 4.0 mg/l and 2,4-dichlorophenoxyacetic acid (2,4-D), at 0.1 mg/l, and shoot tip was cultured on MS supplemented with various concentrations of benzyladenine (BA at the rate of 0.0, 1.0, 2.0 and 4.0 mg/l).The results showed that MS with 4.0 mg/l TDZ cooperate 0.1 mg/l 2,4-D induced the highest percentage callus of young leaf at 80, and MS with 4 mg/l BA induced the highest shoots of shoot tip (8,4 shoot per explants). |
