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In Vitro Propagtion of Venus' Slipper (Paphiopedilum godefroyae (Godefr.) Pfitz.) |
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| รหัสดีโอไอ | |
| Creator | Tharntip Pecharaburanin |
| Title | In Vitro Propagtion of Venus' Slipper (Paphiopedilum godefroyae (Godefr.) Pfitz.) |
| Publisher | Department of Agriculture |
| Publication Year | 2549 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 24 |
| Journal No. | 3 |
| Page no. | 230-246 |
| Keyword | in vitro, propagation, Venus' slipper |
| URL Website | https://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | In vitro propagation of Paphiopedilum godefroyae (Godefr.) Pfitz. was inducted the large number of multiple shoots and conducted at the laboratory of Biotechnology Research and Development Office during 2001-2006. The experiment was divided into two trials. The frist one was studied on the effects of growth regulator of BA (6-bebzyladenine) and NAA (naphthaleneacetic acid) on the plantlets development. The experiment was designed s 4x5 Factorial in CRD with 5 replications. The plantlets were cultured on Murashige and Skoog (MS,1962) medium suplemented with 0, 1, 2 and 3 mg/I BA and 0, 011, 0.3,0.5 and 0.7 mg/I NAA. After 8 months the best growth and development of plantlets was obtained in cultured of MS + BA 2 mg/I + NAA 0.5 mg/I media. The result was completely supported vigorous leaves, roots and 3 shoots per plant initiation. The second trial was studied on colnal propagation or root part and was designed as CRD with 10 replications and 3 treatments. The diameter of expansion roots 2, 3 and 4 cm of 8 months old were cultured on MS+BA 2 mg/I + NAA 0.5 mg/I media. After 10 months in culture, the expansion root 4 cm of diameters gave the highes tnumber at 38.9 shoots per root. The shoots were expcised and cultured on MS+BA 2 mg/I NAA 0.5 mg/I + activated charcoal 2 gm/I media to develop the roots and vigorous plantlets into the large numbers for propagation and conservation under natural condition. |
