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Identification of Selected F1 hybrid durians as promising lines by DNA Amplification Fingerprinting (DAF) |
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| รหัสดีโอไอ | |
| Creator | Songpol Somsri |
| Title | Identification of Selected F1 hybrid durians as promising lines by DNA Amplification Fingerprinting (DAF) |
| Contributor | Suchirat Sakuanrungsirikul, Wanida Ngam-Ngern, Therawut Wongvarat |
| Publisher | Department of Agriculture |
| Publication Year | 2548 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 23 |
| Journal No. | 3 |
| Page no. | 313-330 |
| Keyword | D. zibethinus, F1 hybrids, promising lines, identification, DNA Fingerprinting, DAF |
| URL Website | https://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | The polymerase chain reaction (PCR) โ" based DNA Amplification Fingerprinting (DAF) approach was used to investigate genetic relationships among F1 hybrid durians and their parents including promising lines. Pair wise similarity matrix were developed using band sharing data generated by the selected 12 out of 180 primers such as Operon A-04, A-06, A-09, A-19, A-20, B-01, B-03, B-16, C-06, G-20, S-01 and S-02. These experiments were conducted at Chanthaburi Horticultural Research Centre and Horticulture Research Institute between October 1999 โ" September 2005. In the first experiments with 5 F1 hybrids from the CN x MT cross, there are 31 polymorphisms from a total of 118 produced bands or 26.27%. Second experiment with 120 F1 hybrids from 24 crosses, 166 polymorphisms were identified from a total of 276 produced bands or 60.14%. The third experiments, 156 polymorphic loci from 298 loci or 52.35% were evaluated for 29 promising F1 hybrids identification. Dendrograms were constructed using the unwished pair group method with arithmetic averages (UPGMA), could separate the genotypes of F1 hybrids, promising F1 hybrids and their parents into 4, 2 and 3 group for these experiments, respectively. |
