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Embryogenesis of Citrus reticulata (Keawan and Shokoon) |
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| รหัสดีโอไอ | |
| Creator | Jaruwan Chartisathian |
| Title | Embryogenesis of Citrus reticulata (Keawan and Shokoon) |
| Contributor | Kasedis Distabanjong, Hirun Hirunpradit |
| Publisher | Department of Agriculture |
| Publication Year | 2544 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 19 |
| Journal No. | 1 |
| Page no. | 44-57 |
| Keyword | tissue culture, Keawan and shokoon |
| URL Website | https://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | Embryogenic callus can be induced from immature ovule of Citrus reticulata, Thai cultivars : Som Keawan and Som Shokoon. the higher callus induction frequency can be obtained from fruits at 0.6-0.8 cm diameters. The larger ovule sizes showed higher frequency inducting callus than that of the smaller ones. Callus can be induced by two different kinds of media, Murashige & Tucker basal medium + 500 mg./L malt extract + 2 mg./L BA and Basal medium + 500 mg./L malt extract + 1 mg./L kinetin + 0.1 mg./L IAA. Callus can be proliferated in both liquid and solid basal media of Murashige & Tucker. Development media of Som Keawan and Som shokoon were supplemented with 5% galactose or 5% lactose. Somatic embryos can be regenerated with normal shoots and roots in the media containing 1.0 mg./L GA3 |