Embryogenesis of Citrus reticulata (Keawan and Shokoon)
รหัสดีโอไอ
Creator Jaruwan Chartisathian
Title Embryogenesis of Citrus reticulata (Keawan and Shokoon)
Contributor Kasedis Distabanjong, Hirun Hirunpradit
Publisher Department of Agriculture
Publication Year 2544
Journal Title Thai Agricultural Research Journal
Journal Vol. 19
Journal No. 1
Page no. 44-57
Keyword tissue culture, Keawan and shokoon
URL Website https://at.doa.go.th/journal
Website title Thai Agricultural Research Journal
ISSN 0125-8389
Abstract Embryogenic callus can be induced from immature ovule of Citrus reticulata, Thai cultivars : Som Keawan and Som Shokoon. the higher callus induction frequency can be obtained from fruits at 0.6-0.8 cm diameters. The larger ovule sizes showed higher frequency inducting callus than that of the smaller ones. Callus can be induced by two different kinds of media, Murashige & Tucker basal medium + 500 mg./L malt extract + 2 mg./L BA and Basal medium + 500 mg./L malt extract + 1 mg./L kinetin + 0.1 mg./L IAA. Callus can be proliferated in both liquid and solid basal media of Murashige & Tucker. Development media of Som Keawan and Som shokoon were supplemented with 5% galactose or 5% lactose. Somatic embryos can be regenerated with normal shoots and roots in the media containing 1.0 mg./L GA3
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