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The In vitro Effect of a Crude Extract from Artocarpus lakoocha Roxb on Paramphistomum cervi |
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| รหัสดีโอไอ | |
| Creator | 1. Mananya Preyavichyapugdee 2. Manaw Sangfuang 3. Narin Preyavichyapugdee 4. Yupa Pootaeng-on 5. Prasert Sophon |
| Title | The In vitro Effect of a Crude Extract from Artocarpus lakoocha Roxb on Paramphistomum cervi |
| Publisher | Silpakorn University Research and Development Institute |
| Publication Year | 2560 |
| Journal Title | Silpakorn University Science and Technology Journal |
| Journal Vol. | 11 |
| Journal No. | 1 |
| Page no. | 36-46 |
| Keyword | Artocarpus lakoocha, Paramphistomosis, Anthelmintic drug, Organic farming |
| ISSN | 2586-842x |
| Abstract | The present study was carried out to evaluate the anthelminthic activity of the extract from Artocarpus lakoocha Roxb (Traditional name: "Puag-Haad" (PH)) against rumen fluke, Paramphistomum cervi. The parasites were incubated in M199 medium containing either extract of PH or albendazole (ABZ) at 250, 500, 750, 1,000, 2,000 ตg/ml, for 3, 6, 12 and 24 h. The efficacy of the extracts or ABZ was assessed by using relative motility (RM) assay which was determined on the basis of comparing the loss of spontaneous movement and/or death of the trematodes between treated and control group. The trematodes were further observed under scanning electron microscopy (SEM). PH expressed the potential in killing the trematode and the maximum efficacy was exhibited at 2,000 ตg/ml which was higher and had significantly different RM and SI values than ABZ (p<0.05).Through observations by the SEM it was found that the PH also caused more damage on the tegument of P. cervithan ABZ, while the sequence of tegumental alterations was similar, i.e. comprising of swelling, blebbing, related to concentration of the crude extract and time of incubation. The results obtained in the present SEM-based study established the anthelmintic activity of PH against P. cervi. |
