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Isolation of Thermotoga spp. and Fervidobacterium spp., and Characterization of 16S rRNA Genes of Order Thermotogales: Unique Lineage of Hyperthermophiles Thriving in 3 Hot Springs in Thailand |
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| รหัสดีโอไอ | |
| Creator | 1. Porranee Keawram 2. Veeranun Pongsapukdee 3. Wirojne Kanoksilapatham |
| Title | Isolation of Thermotoga spp. and Fervidobacterium spp., and Characterization of 16S rRNA Genes of Order Thermotogales: Unique Lineage of Hyperthermophiles Thriving in 3 Hot Springs in Thailand |
| Publisher | Silpakorn University Research and Development Institute |
| Publication Year | 2559 |
| Journal Title | Silpakorn University Science and Technology Journal |
| Journal Vol. | 10 |
| Journal No. | 1 |
| Page no. | .9-20 |
| Keyword | Fervidobacterium, Hot spring, Hyperthermophile, Thermotoga, Thermotogales |
| ISSN | 1905-9159 |
| Abstract | Order Thermotogales comprise mostly strictly anaerobic thermophilic and hyperthermophilic bacteria which thrive in high temperature habitats throughout the world. In order to study diversity of hyperthermophiles in Thailand's hot springs, culture dependent technique was performed at 80 o C. Pure cultures of 19 isolates obtained from 3 hot springs were characterized based on 16S rRNA genes of order Thermotogales. Twelve and 7 isolates were identified as deepest blanches within two clades formed by divergent encapsulated rod shaped bacteria belonging to Fervidobacterium and Thermotoga, respectively. Phylogenetic evidences suggest that the Thailand strains from both branches are possible candidates for two novel species. Closely related strains within both genera were identified regardless of their habitats and years of sample collection, implying that these native inhabitants found in Thailand's hot springs might have evolved from a unique common ancestor. Because all isolates require extremely high temperatures for growth, these isolates are likely sources of genes encoding numerous thermostable enzymes |
