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Somatic Embryogenesis Induction from Protocorm-like Bodies and Leaf Segments of Dendrobium Sonia 'Earsakul' |
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| รหัสดีโอไอ | |
| Creator | 1. Kanjana Juntada 2. Sudawadee Taboonmee 3. Pimjai Meetum 4. Sirinnapa Poomjae 5. Pantipa Na Chiangmai |
| Title | Somatic Embryogenesis Induction from Protocorm-like Bodies and Leaf Segments of Dendrobium Sonia 'Earsakul' |
| Publisher | Silpakorn University Research and Development Institute |
| Publication Year | 2558 |
| Journal Title | Silpakorn University Science and Technology Journal |
| Journal Vol. | 9 |
| Journal No. | 2 |
| Page no. | 9. - 19 |
| Keyword | Dendrobium, Plant growth regulators (PGRs), Protocorm-like bodies (PLBs), Somatic embryogenesis |
| ISSN | 1905-9159 |
| Abstract | This study aimed to evaluate effects of types and concentrations of plant growth regulators in a halfstrength Murashige and Skoog medium on somatic embryogenesis from protocorm-like bodies (PLBs) and leaf segments of Dendrobium Sonia 'Earsakul' cultured for 8 weeks. It was found that media containing thidiazuron (TDZ) either at 0.1, 0.3 and 1.0 mg/L alone or in combinations with 0.1 mg/L naphthaleneacetic acid (NAA) or 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) provided no significant differences in percentage of somatic embryogenesis (38.18 49%) from PLBs. Half strength MS supplemented with TDZ either at 1 and 3 mg/L alone or combination with 0.1 mg/L NAA provided higher percentage of somatic embryo formation and number of somatic embryo per explant more than supplemented with 0.1 mg/L NAA alone. Low percentage of somatic embryogenesis was found from leaf segments. Leaf explants with the dorsal surface in contact with a medium provided higher somatic embryogenesis (0-18.75%) than those obtained from the explants with the ventral surface contact with culture medium (0.89-8.04%). Furthermore, basal segments of leaves with ventral surface in contact with a medium showed higher somatic embryogenesis and number of embryos (1.79-16.07% and 0.2-0.85, respectively) than those obtained from apical segments (0-1.78% and 0 0.3, respectively). There was a significant difference in somatic embryogenesis (0-18.75%) of a leaf when a dorsal surface was placed on the culture medium. |
