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Regeneration of Adventitious Shoots from Callus and Leaf Explants in Jatropha curcas L. 'Phetchaburi' |
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| รหัสดีโอไอ | |
| Creator | 1. Pantipa Na Chiangmai 2. Yupa Pootaeng-on 3. Pimjai Meetum 4. Kanokwan Kamkajon 5. Waraporn Yuiam 6. Narumon Rungphan 7. Panwadee Ninkaew |
| Title | Regeneration of Adventitious Shoots from Callus and Leaf Explants in Jatropha curcas L. 'Phetchaburi' |
| Publisher | Silpakorn University Research and Development Institute |
| Publication Year | 2558 |
| Journal Title | Silpakorn University Science and Technology Journal |
| Journal Vol. | 9 |
| Journal No. | 1 |
| Page no. | 28-39 |
| Keyword | Physic nut, Plant growth regulators, Shoot regeneration, Tissue culture explants |
| ISSN | 1905-9159 |
| Abstract | The effect of cytokinins and auxins was evaluated in inducing a physic nut (Jatropha curcas L.) callus while the effect of the combinations of cytokinin and either indole 3-butyric acid (IBA) or some growth additives was determined in regenerating adventitious shoot from a leaf segment. The results showed MS medium containing only 0.1 or 1.0 mg/l thidiazuron (TDZ) was the most effective medium for inducing morphogenic callus. MS medium supplemented with 2.0 mg/l 6-benzylaminopurine (BA) and 0.5 mg/l indole-3-acetic acid (IAA) gave the highest shoot regeneration percentage and shoot height from the callus. On the other hand, the MS medium supplemented with 0.5 mg/l BA and 1.0 mg/l IBA was the most effective medium for adventitious shoot regeneration and shoot height from the leaf segments. |
