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Mutation Induction in Physic Nut (Jatropha curcas L.) by Colchicine Treatments |
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| รหัสดีโอไอ | |
| Creator | 1. Pantipa Na Chiangmai 2. Yupa Pootaeng-on 3. Pimjai Meetum 4. Neeranoot Jankomon 5. Daungruethai Muangnoi 6. Duanpen Kitthip |
| Title | Mutation Induction in Physic Nut (Jatropha curcas L.) by Colchicine Treatments |
| Publisher | Silpakorn University Research and Development Institute |
| Publication Year | 2557 |
| Journal Title | Silpakorn University Science and Technology |
| Journal Vol. | 8 |
| Journal No. | 2 |
| Page no. | 28-39 |
| Keyword | Aneuploid, Energy plant, Genetic variability, Mixoploid, Ploidy chromosome level |
| ISSN | 1905-9159 |
| Abstract | The objective of this study aimed to evaluate the genetic variation in physic nut (Jatropha curcas L.) by colchicine induction in two experiments. In Experiment 1, inflorescences of physic nut were treated under different colchicine concentrations by dropping the solution directly at the stage that the frequency of male flower at microspore stage. In Experiment 2, seeds were soaked in different concentrations of colchicine at various durations.In Experiment 1 and 2, the results showed that the increasing of colchicine concentration likely to decreased size of guard cells, plant height and stomatal density. However, significant different among those parameters was not observed. The ploidy level determined from leaf cells of plants by flow cytometry in both experiments showed almost of plants were disomic (2x). However, in experiment 1, incomplete aneuploidy or mixoploid plants that mixing between trisomic (2x + 1) (at 0 mM colchicine) and tetrasomic (2x + 2) (at 2.5 mM colchicine) with disomic were also found. In experiment 2, incomplete aneuploid plants were composed of trisomic, tetrasomic and pentasomic (2x + 3) with disomic. The mixoploid plant of disomic, trisomic and hexasomic (2x + 4) was also detected when concentrations of colchicine and times for soaking duration were increased. |
