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Extended-spectrum beta lactamase and carbapenemaseproducing Klebsialla spp. in urine and fecal samples obtainedfrom hospitals and communities in Lagos, Nigeria |
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| รหัสดีโอไอ | |
| Creator | Tenny Obiageli Egwuatu |
| Title | Extended-spectrum beta lactamase and carbapenemaseproducing Klebsialla spp. in urine and fecal samples obtainedfrom hospitals and communities in Lagos, Nigeria |
| Contributor | Godwin Ochuko Orkeh |
| Publisher | Faculty of Science and Technology, Suan Sunandha Rajabhat University |
| Publication Year | 2562 |
| Journal Title | Suan Sunandha Science and Technology Journal |
| Journal Vol. | 6 |
| Journal No. | 1 |
| Page no. | 6 to 14 |
| Keyword | Extended Spectrum Beta-Lactamase, Urinary Tract Infections, Carbapenemase, Klebsiella spp |
| URL Website | www.ssstj.sci.ssru.ac.th |
| Website title | Suan Sunandha Science and Technology Journal (SSSTJ) |
| ISSN | 2351-0889 |
| Abstract | The use of beta-lactams has tremendously increased since the discovery of antibiotics. This has led to the emergenceof certain resistant genes such as Extended Spectrum Beta-Lactamase (ESBL) which confer resistance to thirdgeneration cephalosporins. The objective of this study was to determine the prevalence of Extended Spectrum BetaLactamase (ESBL) genes, Carbapenem resistance genes (blaKPC, blaOXA and IMP) and outer membrane porinsgenes (OMP-35, OMP-36, and OMP-36N) from different hospitals and laboratories in Ikeja-Lagos, Nigeria. A totalof 177 bacterial isolates were collected between May 2017 to July 2017 from patients with urinary tract infections(UTI) and gastroenteritis. They were identified biochemically and investigated for ESBL and Carbapenemaseproduction using phenotypic Double Disk Synergy Test (DDST) and Modified Hodges' Test respectively.Antibiotics susceptibility profile was also investigated. Multiplex PCR was used to detect the genes responsible forthe resistant genes. Out of 177 bacterial isolates, 47 (26.6%) were identified as Klebsiella spp and 17 (36.1%) wereESBL positive and then 5 (29.4%) were positive for carbapenem resistance. Multiplex PCR revealed that 3 (27.3%)possessed both blaCTX-M and blaSHV genes, 6 (54.5%) possessed only blaCTX-M gene while only 2 (18.2%) possessedonly blaSHV gene. Also, 13 (76.5%) possessed only blaKPC gene. However, blaTEM as well as IMP, OXA-48, OMP35, OMP 36 and OMP 36N genes were not detected. This study revealed that antibiotic resistance is on the rise andpreventive measures should be put in place by both government and health care providers to curtail this trend. |
