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B-glucosidase enzyme screening from various parts of Tabebuia argentea |
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| รหัสดีโอไอ | |
| Creator | Chariwat Pitsanuwong |
| Title | B-glucosidase enzyme screening from various parts of Tabebuia argentea |
| Contributor | KanokornWechakorn |
| Publisher | Faculty of Science and Technology, Suan Sunandha Rajabhat University |
| Publication Year | 2561 |
| Journal Title | Suan Sunandha Science and Technology Journal |
| Journal Vol. | 5 |
| Journal No. | 2 |
| Page no. | 6 to 10 |
| Keyword | Bglucosidase, Tabebuia aurea, p-nitrophenyl-D-glucopyranoside |
| URL Website | www.ssstj.sci.ssru.ac.th |
| Website title | Suan Sunandha Science and Technology Journal (SSSTJ) |
| ISSN | 2351-0889 |
| Abstract | The aim of this work is the screening for searching the novel ?glucosidase enzyme from various parts of Tabebuia argentea; flower, flower bud, shoot, seed shank and seed. The enzyme extracted from the samples by using appropriated buffer. The ammonium sulfate ((NH4)2SO4) salt precipitation in the different salt concentration was used to initial fractionated purification steps. The monitoring of enzyme activity by using hydrolysis reaction of glycosidic bond by using p-nitrophenyl-D-glucopyranoside (pNPG) as an enzyme substrate. The UV-vis spectroscopy used to detect the corresponding p-nitrophenylate product under basic condition at 405 nm. The enzyme activity via pNPG hydrolysis of seeds extract responds toward around 15-fold over the other part extracts of Tabebuia argentea, follow by selected enzyme fraction of seeds extract to subject to optimum temperature study, the temperature for the best enzyme activity is 30-40 ?C. The highest enzyme activity fraction will be used for further purification and enzymatic properties test before apply to be a biocatalyst in biological process. |
