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Protective effects of lotus stamen extracton UV-B-irradiated skin cells activities |
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| รหัสดีโอไอ | |
| Creator | 1. Preeyawass Phimnuan 2. Racharat Rukchay 3. Nichanan Tiraravesit 4. Sombat Wanauppathamkul 5. Suppisak Chaturonratsamee 6. Prapadsorn Pittayanurak 7. Vichai Reutrakul 8. Jarupa Viyoch |
| Title | Protective effects of lotus stamen extracton UV-B-irradiated skin cells activities |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2563 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 42 |
| Journal No. | 2 |
| Page no. | 383-390 |
| Keyword | lotus stamen, protective effect, melanogenesis inhibitory activity, anti-oxidant activity, anti-inflammatory activity |
| URL Website | https://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | Traditionally, Nelumbo nucifera has been used as a tonic and stimulant. The aim of this study was to clarify aprotective effect regarding UV-B induced inflammation and melanogenesis activities of the crude extract of the dried lotusstamen extract. The compounds of the extract were isolated by column chromatography. The anti-oxidant EC50 value of the crudeextract was 0.0353 mg/mL while the EC50 values of the isolated compounds of kaempferol and kaempferol 3-O-?-Dglucopyranoside were 0.0030 and 0.0077 mg/mL, respectively, which were observed by DPPH assay. The tyrosinase inhibitoryactivity was tested by tyrosinase enzyme. The anti-tyrosinase IC50 value of the crude was 0.0192 mg/mL while the isolatedkaempferol was 0.0496 mg/mL. To determine the anti-inflammatory activity, the molecular targets for the extract action wereTNF-? and IL-6. The release of TNF-? and IL-6 was significantly reduced in UV-B-induced keratinocytes pretreated with thecrude extract at concentrations from 25 to 100 ?g/mL. Moreover, the crude extract was found to inhibit the melaninoverproduction of B16F1 induced by ?-MSH. |
