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In Vitro Self and Cross Pollinated Seed Culture of Nymphaea rubra 'Maeploi', A Night Blooming Tropical Waterlily Hybrid (Nymphaeaceae) from Thailand |
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| รหัสดีโอไอ | |
| Creator | Itsaraphong Khaenthong |
| Title | In Vitro Self and Cross Pollinated Seed Culture of Nymphaea rubra 'Maeploi', A Night Blooming Tropical Waterlily Hybrid (Nymphaeaceae) from Thailand |
| Contributor | Ngarmnij Chuenboonngarm, Atchara Muengkrut |
| Publisher | สถาบันวิจัยและพัฒนา มหาวิทยาลัยเทคโนโลยีราชมงคลธัญบุรี |
| Publication Year | 2564 |
| Journal Title | วารสารวิจัย มหาวิทยาลัยเทคโนโลยีราชมงคลธัญบุรี |
| Journal Vol. | 20 |
| Journal No. | 1 |
| Page no. | 123-133 |
| Keyword | Nymphaeaceae, interspe-cific hybridization, hand pollination, germination, ex situ conservation |
| URL Website | https://ph01.tci-thaijo.org/index.php/rmutt-journal/index |
| Website title | https://www.tci-thaijo.org |
| ISSN | 2651-2290 |
| Abstract | This research was aimed to develop culture medium en-hancing the germination of self and cross-pollinated seeds in Nym-phaea rubra 'Maeploi' and Nymphaea pubescens Willd. The self-pollinated seeds of N. rubra 'Maeploi' were investigated for surface sterilization method, seed culture medium and light conditions. The self-pollinated seeds were surface sterilization with 20% (v/v) sodi-um hypochlorite (NaOCl) at various times (5, 10, 15 and 20 min), fol-lowed by the second surface sterilization with NaOCl at different concentrations (5, 10, 15 and 20% (v/v)) for 10 min. MS medium at different strengths (MS, 1/2MS, 1/4MS, 1/8MS) was also studied to rescue the cross-pollinated seeds, compared to control. The results showed that the fruit set percentages of N. pubescens x N. rubra 'Maeploi' and N. rubra 'Maeploi' x N. pubescens were low at 5% and 3.33%, respectively. The N. rubra 'Maeploi' self-pollinated seeds were soaked with 20% (v/v) NaOCl for 5 min, followed by the sterilization with 5% (v/v) NaOCl for 10 min showed without the mi-crobial contamination. Moreover, the suitable medium for N. rubra 'Maeploi' self-pollinated seeds was 1/8MS semi solid medium under dark condition. The germinated seedlings were developed shoots (60%), immature leaves (51.67%), and roots (48.33%) after 4 weeks of culture. Germination percentages of N. rubra 'Maeploi' and N. pu-bescens self-pollinated seeds cultured on 1/8MS were 51.67 higher than those of cross-pollinated seeds of N. pubescens x N. rubra 'Maeploi' and N. rubra 'Maeploi' x N. pubescens (35 and 31.67%, respectively). |
