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Novel enzymes produced by actinobacteria growing on seaweed |
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| รหัสดีโอไอ | |
| Creator | Thi Nhu Thuong Nguyen*, Christopher M. M. Franco and Stephen Barnett |
| Title | Novel enzymes produced by actinobacteria growing on seaweed |
| Publisher | Mahsarakham University |
| Publication Year | 2561 |
| Journal Title | Research & Knowledge |
| Journal Vol. | 4 |
| Journal No. | 1 |
| Keyword | Actinobacteria, polysaccharide-degrading enzymes, seaweed |
| ISSN | 2408-204X |
| Abstract | Actinobacteria were screened for their ability to produce seaweed polysaccharide-degradingenzymes. Streptomyces strains FA24 and FA56 grown on seaweed which could both produce alginatelyase. The polysaccharide-degrading enzymes were precipitated with ammonium sulphate and thenpurified using dialysis and anion exchange chromatography. Polysaccharide-degrading enzymes fromStreptomyces sp. FA24 had a molecular mass range of 15 37 kDa while enzymes from Streptomycessp. FA56 had an apparent molecular mass of 23 kDa. The optimum pH for activity of polysaccharidedegradingenzymes from Streptomyces spp. FA24 and FA56 against sodium alginate were 8.0 and 7.5,respectively. These enzymes of both strains also showed maximum activity at 30oC. Streptomyces spp.FA24 and FA56 were observed as having a 95% and 99% 16S rRNA gene sequence similarity,respectively, to previously recorded sequences of type strains of actinobacteria isolates, indicating thatStreptomyces sp. FA24 could be novel species. The enzymes purified from Streptomyces spp. FA24and FA56 grown on Bull Kelp powder may be novel alginate lyases. |
