|
Cytotoxic activity of marcanine C and some other aminoquinone derivatives against MCF-7 cells |
|---|---|
| รหัสดีโอไอ | |
| Creator | 1. P. Thanuphol 2. E. Noulsri 3. K. Suwanborirux 4. N. Soonthornchareonnon 5. K. Pattanapanyasat |
| Title | Cytotoxic activity of marcanine C and some other aminoquinone derivatives against MCF-7 cells |
| Publisher | Faculty of Pharmacy, Mahidol University |
| Publication Year | 2559 |
| Journal Title | Mahidol University Journal of Pharmaceutical Sciences |
| Journal Vol. | 43 |
| Journal No. | 4 |
| Page no. | 203-210 |
| Keyword | marcanine C,1-azaanthraquinones,apoptosis,cell cycle,caspase-3,Goniothalamus |
| ISSN | 0125-1570 |
| Abstract | Alkaloids from Goniothalamus have been isolated and renowned for their cytotoxicity. Recently, there has been an increment of interest to study on their chemical structures and the molecular mechanisms of action. The study herein, reported the cytotoxic effects of some Goniothalamus alkaloids in MCF-7 human breast adenocarcinoma cells as well as NB4 acute promyelocytic leukemia cells. The effects of marcanine B, C, and other structural-related compounds namely liriodenine and 5-hydroxy-3 amino-2-aceto-1,4-naphthoquinone (HAANQ) on apoptosis, caspase-3 activation and cell cycle arrest were evaluated by flow cytometry. We found that, marcanine C showed the most prominent apoptosis inducing effect on both MCF-7 and NB4 cells. As proven by the result of caspase-3 activation, marcanine C 7.5 ?M for 24 hr induced more than 23% the activation of caspase-3 on MCF-7 cells. Furthermore, marcanine C caused cell cycle arrest in the G1 phase along with low viability of the cells in S and G2/M phases suggesting the prevention of DNA synthesis and the consequent apoptosis. In summary, marcanine C exerted the most prominent cytotoxic effect among all test compounds. This study is the first to report the effects of marcanine C on apoptosis induction in breast cancer cells via caspase-3 activation and the cell cycle arrest in G1 phase. |
