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Chemical profiles and chemometric analysis of selected Leucobryum species (Bryophyta, Leucobryaceae) from Thailand |
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| รหัสดีโอไอ | |
| Creator | Ekaphan Kraichak |
| Title | Chemical profiles and chemometric analysis of selected Leucobryum species (Bryophyta, Leucobryaceae) from Thailand |
| Contributor | Patsakorn Tiwutanon, Natthawadi Wongthet, Srunya Vajrodaya |
| Publisher | Maejo University |
| Publication Year | 2567 |
| Journal Title | Maejo International Journal of Science and Technology |
| Journal Vol. | 18 |
| Journal No. | 2 |
| Page no. | 157 |
| Keyword | Leucobryum species, bryophytes, mosses, chemical profiles, cluster analysis, Thailand |
| Website title | Maejo International Journal of Science and Technology |
| ISSN | 1905-7873 |
| Abstract | Leucobryum is a moss genus that exhibits various bioactivities. However, the identification of Leucobryum species with morphology alone remains difficult. Chemical profile analysis provides additional tools for plant classification. This method uses chemical similarities to identify the differences among some plants, especially the different varieties of plant species. The objective of this study was to obtain the chemical profiles and use chemometrics to identify selected Leucobryum species found in Thailand. Lipophilic extracts from 18 samples of five taxa were analysed with thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). Principal component analysis (PCA) and hierarchical cluster analysis were used to investigate the similarities of the chemical profiles. Permutational multivariate analysis of variance (PERMANOVA) was performed to determine the difference among taxa. The taxonomic identities were also verified with a molecular marker. Morphological and molecular identification were consistent for five taxa. The TLC profile could separate only the genus of the sample, while HPLC chromatograms showed separation among the Leucobryum taxa and consistent patterns within the same species. In addition, the chemical profiles of Leucobryum species were separated at the species level in PCA. The PERMANOVA showed significantly different profiles among the species (P value = 0.002). Therefore, this work illustrates the potential of chemometrics as supporting evidence for species delimitation in the moss genus Leucobryum. |
