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Anti-cancer effects of astaxanthin extract from wild-type and hyperproducing Xanthophyllomyces dendrorhous mutant on breast cancer cells of dissimilar estrogen receptor status |
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| รหัสดีโอไอ | |
| Creator | Chew Ai Lan |
| Title | Anti-cancer effects of astaxanthin extract from wild-type and hyperproducing Xanthophyllomyces dendrorhous mutant on breast cancer cells of dissimilar estrogen receptor status |
| Contributor | Khaw Shin Yuan, Neshalini Sathiabalan, Ong Khang Wei |
| Publisher | Maejo University |
| Publication Year | 2566 |
| Journal Title | Maejo International Journal of Science and Technology |
| Journal Vol. | 17 |
| Journal No. | 1 |
| Page no. | 24 |
| Keyword | astaxanthin, Xanthophyllomyces dendrorhous, breast cancer cells, dissimilar estrogen receptor |
| Website title | Maejo International Journal of Science and Technology |
| ISSN | 1905-7873 |
| Abstract | The effects of astaxanthin extracts from wild-type and mutant Xanthophyllomyces dendrorhous on MCF7 and MDA-MB-231 breast cancer cells were examined. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assays prove that the wild-type and mutant extracts are non-toxic towards non-cancerous MCF-10A cells but exhibit a growth-inhibitory effect on MCF-7 and MDA-MB-231 cells in a dose-dependent manner. The mutant extract shows a lower IC50 and is more effective in inhibiting MCF-7 and MDA-MB-231 cells. The IC50 values did not exceed the recommended limit of 30 g/ml. MCF-7 and MDA-MB-231 cells lose their shape after treatment with the wild-type or mutant extract and apoptosis hallmarks develop in a time-dependent manner. Flow cytometry analysis signifies apoptosis induction by both extracts in MCF-7 and MDA-MB-231 cells in a cell-type-dependent manner. Cell cycle arrest is observed at the S phase and G2/M phase in MCF-7 cells treated with wild-type extract and at the G2/M phase when treated with mutant extract. In MDA-MB-231 cells, cell cycle arrest is observed at the S phase for both extract treatments. Reactive oxygen species (ROS) analysis shows 2-fold ROS accumulation in MCF-7 and MDA-MB-231 cells after treatment with both extracts. Wound healing results demonstrate that the mutant extract exhibits better migration inhibition in MCF-7 and MDA-MB-231 cells than the wild type extract. Both extracts give better inhibition on MCF-7 cells than MDA-MB-231 cells. |
