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Design and optimisation of enzymatic glucose assay in mesofluidic chemiluminescence device with glucose oxidase immobilised on pencil lead |
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| รหัสดีโอไอ | |
| Creator | 1. Sakchai Satienperakul 2. Watcharin Seeramad 3. Lori Shayne T. Alamo 4. Tanin Tanguaram |
| Title | Design and optimisation of enzymatic glucose assay in mesofluidic chemiluminescence device with glucose oxidase immobilised on pencil lead |
| Publisher | Maejo University |
| Publication Year | 2559 |
| Journal Title | Maejo International Journal of Science and Technology |
| Journal Vol. | 10 |
| Journal No. | 1 |
| Page no. | 66 |
| Keyword | glucose biosensor,glucose oxidase,meso-fluidic device,chemiluminescence detection |
| ISSN | 1905-7873 |
| Abstract | A simple meso-fluidic chemiluminescence device was developed for glucose determination using an enzymatic assay. A gold nanoparticles/glucose oxidase bioreactor was constructed by immobilising glucose oxidase enzyme on a piece of pencil lead and packing it into a channel. Poly(methyl methacrylate) and poly(dimethylsiloxane) were used for the fabrication of a sandwich-type meso-fluidic device. A chemiluminescence measurement set-up was employed to monitor the oxidation processes in the meso-fluidic chip. The enzymatic glucose assay involves the oxidation of glucose to generate hydrogen peroxide followed by luminol/Co2+ chemiluminescence detection. Reagent concentration, flow rate, sample volume and photomultiplier tube applied voltage were thoroughly optimised. A linear relation exists for glucose concentration between 0.5 ? 10-5 - 1.2 ? 10-3 M (r2= 0.9946). The detection limit (3?) was found to be 1.0 ? 10-6 M. The proposed system was successfully applied for quantitative analysis of glucose in biological fluids and energy drink samples. The meso-fluidic data were validated with standard spectrophotometric or HPLC methods. |
