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COMPARING METHODS FOR THE PRESERVATION OF STREPTOMYCES TM32 TO DEVELOP IT INTO A BIOLOGICAL PRODUCT FOR INHIBITING SOIL-BORNE PLANT PATHOGENS |
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| รหัสดีโอไอ | |
| Creator | Nareeluk Nakaew |
| Title | COMPARING METHODS FOR THE PRESERVATION OF STREPTOMYCES TM32 TO DEVELOP IT INTO A BIOLOGICAL PRODUCT FOR INHIBITING SOIL-BORNE PLANT PATHOGENS |
| Contributor | Chutaporn Mengerd, Napatsawan Panyasa, Parphatson Jomngam, Boonruang Khamsri, Juangjun Jumpathong, Pairote Wongputtisin |
| Publisher | Pibulsongkram Rajabhat University |
| Publication Year | 2567 |
| Journal Title | Life Sciences and Environment Journal |
| Journal Vol. | 25 |
| Journal No. | 1 |
| Page no. | 225-236 |
| Keyword | biological products, biological control of plant diseases, plant pathogenic fungi, streptomyces |
| URL Website | https://ph01.tci-thaijo.org/index.php/psru/index |
| Website title | Life Sciences and Environment Journal |
| ISSN | 2773-9201 |
| Abstract | Prevention and control of pests by biological products is one option that can reduce or replace the use of agricultural chemicals to produce safe products. Streptomyces TM32 can inhibit various types of plant pathogenic fungi and produce substances that promote plant growth. Therefore, this research aims to develop a biological product formula to inhibit the fungal growth that causes disease on plant roots from Streptomyces TM32 by comparing the methods of preserving them for development into four biological product formulas (1. Cooked rice, 2. wheat flour and Dolomite (6:3), 3. Talcum and CMC (2:1) and 4. Rice flour, Palm oil and Sucrose (87:3:10) volume-by-volume ratio). The survival of biological products was tested in each formula under temperature storage conditions at 10 and 35oC for 14, 28, and 42 days with the spread plate technique. The results found that formula 1 (Cooked rice), when stored at 35oC for 14 days, had the highest amount of germs remaining, equal to 6.71 ± 0.08 log CFU/g. Therefore, formula 1 was used to test the effectiveness of biological products in inhibiting fungal growth in plants. The experimental set was divided into four sets: unsterilized soil with no added biological agents (Experimental set 1) and with biological products (Experimental set 2); sterilized soil without added biological agents (Experimental set 3) and with biological products added (Experimental set 4). The experiment was performed in a relative humidity condition of 70%, temperature of 25oC and watering every 2 days for 35 days to compare germination percentages and to compare average height of plant (chili plant). The results showed that the second experimental set had the highest germination rate (80%). However, the average height of chili plants after 35 days in this experimental set was lower than that of the control set that had not been inoculated (Experimental sets 1 and 3). |
